Product name: Goat α-Human IgG, Horseradish peroxidase (HRP) labeled
Host species: Goat
Target species: Human
Specificity: No cross-reaction with human IgM and IgA in ELISA No cross-reaction with albumin fraction of human serum. No cross-reaction with albumin fraction of other animal species.
Tested applications: Western Blot and ELISA
Immunogen: Human IgG
Storage instructions: Shipped at 4°C, Store at 4°C
Storage buffer: 50% Glycerol, 0.58% Sodium chloride, 0.164% Sodium phosphate pH 7.50
Concentration: 1 mg/ml
Purity: Immunogen affinity purified
Purification Notes: From the crude polyclonal the crossreactive antibodies were extracted by incubation with Sepharose bound human IgA and IgM. Specific antibodies were absorpted by incubation with Sepharose bound human IgG. Specific antibodies were eluted by acidic buffer at pH 2.5 followed by neutralization and dialysis. After repeated binding with immobilized human IgG a minimum of 65% protein bound.
Conjugation Notes: The purified polyclonal was conjugated to horseradish peroxidase according to the periodate method followed by gel filtation and ion exchange chromatography to clear unbound conjugate.
Recommended Dilutions: Below are the recommended starting dilutions; optimal dilutions/concentrations and should be determined by the end user:
1:1000 – 1:5000 Chemiluminescent detection
1:1000 – 1:2000