H3F3A Antibody, HRP conjugated

$210.00$377.00

Description

Aliases

H3F3A, H3.3A, H3F3, PP781, H3F3B, H3.3B

Antibody Type

Polyclonal Antibody

Species

Human

Uniprot ID

P84243

Immunogen

Recombinant human Histone H3.3 protein (2-136AA)

Raised In

Rabbit

Species Reactivity

Human

Tested Applications

ELISA;Not yet tested in other applications.

Background / Function

Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Isotype

IgG

Conjugate

HRP

Storage Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.

Purity

Caprylic Acid Ammonium Sulfate Precipitation purified

Research

Epigenetics and Nuclear Signaling

Literature

[1]”ZMYND11 links histone H3.3K36me3 to transcription elongation and tumour suppression.”Wen H., Li Y., Xi Y., Jiang S., Stratton S., Peng D., Tanaka K., Ren Y., Xia Z., Wu J., Li B., Barton M.C., Li W., Li H., Shi X.Nature 508:263-268(2014). [2]”Regulat

Additional information

Size

50μg, 100μg

Certificate of Analysis