H3/a, H3/c, H3/d, H3/f, H3/h
Swiss-Prot#:P68431 NCBI Gene#:8351 NCBI Protein#:
Peptide sequence around Di-Methylation site of lysine 27(A-R-K(di-methyl)-S-A) derived from Human Histone H3.
Human Mouse Rat
WB IHC Recommended dilution: Predicted MW: 17kdWestern Blot: 1:500~1000
Background / Function
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.