Histone H3 (Di-Methyl-Lys27) Antibody

$454.00

Cat. No.: 38869 Categories: ,

Description

Aliases

H3/a, H3/c, H3/d, H3/f, H3/h

Antibody Type

Polyclonal Antibody

Uniprot ID

Swiss-Prot#:P68431 NCBI Gene#:8351 NCBI Protein#:

Immunogen

Peptide sequence around Di-Methylation site of lysine 27(A-R-K(di-methyl)-S-A) derived from Human Histone H3.

Raised In

Rabbit

Species Reactivity

Human Mouse Rat

Tested Applications

WB IHC Recommended dilution: Predicted MW: 17kdWestern Blot: 1:500~1000

Background / Function

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Conjugate

Unconjugated

Storage Buffer

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form

liquid

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Modification

Di-Methyl-Lys27

Additional information

Size

100μl

Certificate of Analysis