Description
Aliases
H3/a, H3/c, H3/d, H3/f, H3/h
Antibody Type
Polyclonal Antibody
Uniprot ID
Swiss-Prot#:P68431 NCBI Gene#:8351 NCBI Protein#:
Immunogen
Peptide sequence around Tri-Methylation site of lysine 27(A-R-K(tri-methyl)-S-A) derived from Human Histone H3.
Raised In
Rabbit
Species Reactivity
Human Mouse Rat
Tested Applications
WB IHC IF Recommended dilution: Predicted MW: 17kdWestern Blot: 1:500~1000
Background / Function
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Conjugate
Unconjugated
Storage Buffer
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Modification
Tri-Methyl-Lys27
Additional information
| Size | 100?l |
|---|
