H3 histone antibody; HIST1H3A antibody; Histone cluster 1; H3a antibody
Peptide sequence around phosphorylation site of threonine 118(R-V-T(p)-I-M) derived from Human Histone H3.
Human Rat Mouse
WB Recommended dilution: Western blotting: 1:1000,
Background / Function
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.
PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.