Description
Aliases
I-kappa-B-beta; IKB-B; IKBB; NF-kappa-BIB; NF-kappaB inhibitor beta
Antibody Type
Polyclonal Antibody
Uniprot ID
Swiss-Prot: Q15653
NCBI Protein: NP_001001716.
Immunogen
Peptide sequence around phosphorylation site of serine 23 (L-G-S(p)-L-G) derived from Human IkB-b.
Raised In
Rabbit
Species Reactivity
Human Mouse Rat
Tested Applications
WB IHC Recommended dilution: Predicted MW: 48kd, Immunohistochemistry: 1:50~1:100
Background / Function
Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. However, the unphosphorylated form resynthesized after cell stimulation is able to bind NF-kappa-B allowing its transport to the nucleus and protecting it to further IKBA-dependent inactivation. Association with inhibitor kappa B-interacting NKIRAS1 and NKIRAS2 prevent its phosphorylation rendering it more resistant to degradation, explaining its slower degradation. Shirane, M. et al. (1999) J Biol Chem 274, 28169-28174. DiDonato J, et al. (1996) Mol Cell Biol 16(4): 1295-304
Conjugate
Unconjugated
Storage Buffer
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Modification
Phospho-Ser23
Additional information
| Size | 100?l |
|---|
