I-kappa-B-epsilon; IKBE; IkappaBepsilon; NF-kappa-BIE; NF-kappaB inhibitor epsilon
NCBI Protein: NP_004547.2
Peptide sequence around phosphorylation site of serine 22 (I-E-S(p)-L-R) derived from Human IkB-e.
WB IHC Recommended dilution: Predicted MW: 40kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100,
Background / Function
Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. Inhibits DNA-binding of NF-kappa-B p50-p65 and p50-c-Rel complexes.Shirane M, et al. (1999) J Biol Chem; 274(40): 28169-74Karin, M. and Ben-Neriah, Y. (2000) Annu. Rev. Immunol. ;18, 621-663.Chen, Z.J. et al. (1996) Cell; 84, 853-862.Brown, K. et al. (1995) Science; 267, 1485-1488.
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.