IkB-e(Phospho-Ser22) Antibody

$476.70

Cat. No.: 38801 Categories: ,

Description

Aliases

I-kappa-B-epsilon; IKBE; IkappaBepsilon; NF-kappa-BIE; NF-kappaB inhibitor epsilon

Antibody Type

Polyclonal Antibody

Uniprot ID

Swiss-Prot: O00221
NCBI Protein: NP_004547.2

Immunogen

Peptide sequence around phosphorylation site of serine 22 (I-E-S(p)-L-R) derived from Human IkB-e.

Raised In

Rabbit

Species Reactivity

Human Mouse

Tested Applications

WB IHC Recommended dilution: Predicted MW: 40kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100,

Background / Function

Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. Inhibits DNA-binding of NF-kappa-B p50-p65 and p50-c-Rel complexes.Shirane M, et al. (1999) J Biol Chem; 274(40): 28169-74Karin, M. and Ben-Neriah, Y. (2000) Annu. Rev. Immunol. ;18, 621-663.Chen, Z.J. et al. (1996) Cell; 84, 853-862.Brown, K. et al. (1995) Science; 267, 1485-1488.

Conjugate

Unconjugated

Storage Buffer

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form

liquid

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Modification

Phospho-Ser22

Additional information

Size

100?l

Certificate of Analysis