IKK a(Phospho-Thr23) Antibody

$454.00

Cat. No.: 39000 Categories: ,

Description

Aliases

I kappa-B kinase alpha; I-kappa-B kinase 1; IKK-A; IKK-alpha; IKK1

Antibody Type

Polyclonal Antibody

Uniprot ID

Swiss-Prot: O15111
NCBI Protein: NP_001269.3

Immunogen

Peptide sequence around phosphorylation site of threonine 23 (L-G-T(p)-G-G) derived from Human IKK a.

Raised In

Rabbit

Species Reactivity

Human Mouse Rat

Tested Applications

WB IHC Recommended dilution: Predicted MW: 85kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

Background / Function

Acts as part of the IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. As part of the non-canonical pathway of NF-kappa-B activation, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. Also phosphorylates NCOA3. Phosphorylates ‘Ser-10’ of histone H3 at NF-kappa-B-regulated promoters during inflammatory responses triggered by cytokines. Yuan ZQ, et al.(2002) J Biol Chem; 277(33): 29973-82. Ozes ON, et al. (1999) Nature; 401(6748): 82-5.

Conjugate

Unconjugated

Storage Buffer

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form

liquid

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Modification

Phospho-Thr23

Additional information

Size

100?l

Certificate of Analysis