Description
Aliases
I kappa-B kinase alpha; I-kappa-B kinase 1; IKK-A; IKK-alpha; IKK1
Antibody Type
Polyclonal Antibody
Uniprot ID
Swiss-Prot: O15111
NCBI Protein: NP_001269.3
Immunogen
Peptide sequence around phosphorylation site of threonine 23 (L-G-T(p)-G-G) derived from Human IKK a.
Raised In
Rabbit
Species Reactivity
Human Mouse Rat
Tested Applications
WB IHC Recommended dilution: Predicted MW: 85kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Background / Function
Acts as part of the IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. As part of the non-canonical pathway of NF-kappa-B activation, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. Also phosphorylates NCOA3. Phosphorylates ‘Ser-10’ of histone H3 at NF-kappa-B-regulated promoters during inflammatory responses triggered by cytokines. Yuan ZQ, et al.(2002) J Biol Chem; 277(33): 29973-82. Ozes ON, et al. (1999) Nature; 401(6748): 82-5.
Conjugate
Unconjugated
Storage Buffer
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Modification
Phospho-Thr23
Additional information
| Size | 100?l |
|---|
