IRS-1(Phospho-Ser639) Antibody

$454.00

Cat. No.: 38811 Categories: ,

Description

Aliases

IRS-1; IRS1;

Antibody Type

Polyclonal Antibody

Uniprot ID

Swiss-Prot: P35568
NCBI Protein: NP_005535.1

Immunogen

Peptide sequence around phosphorylation site of serine 639 (P-K-S(p)-V-S) derived from Human IRS-1.

Raised In

Rabbit

Species Reactivity

Human Mouse Rat

Tested Applications

WB IHC Recommended dilution: Predicted MW: 180kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100,

Background / Function

May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit Ozes ON, et al. (2001) Proc Natl Acad Sci U S A; 98(8): 4640-4645Tzatsos A, et al. (2006) Mol Cell Biol; 26(1): 63-76Steppan CM, et al. (2005) Mol Cell Biol; 25(4): 1569-1575Batty IH, et al. (2004) Biochem J; 379(Pt 3): 641-651

Conjugate

Unconjugated

Storage Buffer

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form

liquid

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Modification

Phospho-Ser639

Additional information

Size

100?l

Certificate of Analysis