NCBI Protein: NP_005535.1
Peptide sequence around phosphorylation site of serine 639 (P-K-S(p)-V-S) derived from Human IRS-1.
Human Mouse Rat
WB IHC Recommended dilution: Predicted MW: 180kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100,
Background / Function
May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit Ozes ON, et al. (2001) Proc Natl Acad Sci U S A; 98(8): 4640-4645Tzatsos A, et al. (2006) Mol Cell Biol; 26(1): 63-76Steppan CM, et al. (2005) Mol Cell Biol; 25(4): 1569-1575Batty IH, et al. (2004) Biochem J; 379(Pt 3): 641-651
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.