Keratin 8(Phospho-Ser74) Antibody

$454.00

Cat. No.: 39035 Categories: ,

Description

Aliases

CK 8; CK8; CYK8; Cytokeratin endo A; K8

Antibody Type

Polyclonal Antibody

Uniprot ID

Swiss-Prot: P05787
NCBI Protein: NP_002264.1

Immunogen

Peptide sequence around phosphorylation site of serine 74 (L-L-S(p)-P-L) derived from Human Keratin 8 (CK8).

Raised In

Rabbit

Species Reactivity

Human

Tested Applications

WB IF Recommended dilution: Predicted MW: 55kd, Western blotting: 1:500~1:1000, Immunofluorescence: 1:100~1:200

Background / Function

Keratin 8 is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Nakamichi I, et al. (2002) Mol Biol Cell; 13(10): 3441-3451. Prochasson P, et al. (2002) Nucleic Acids Res; 30(15): 3312-3322. Pankov R, et al. (1994) Proc Natl Acad Sci U S A; 91(3): 873-877. Thorey IS, et al. (1993) Mol Cell Biol; 13(11): 6742-6751

Conjugate

Unconjugated

Storage Buffer

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form

liquid

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Modification

Phospho-Ser74

Additional information

Size

100?l

Certificate of Analysis