LIMK-2; kinase LIMK2;
NCBI Protein: NP_001026971.
Peptide sequence around phosphorylation site of threonine 505 (R-Y-T(p)-V-V) derived from Human LIMK2.
Human Mouse Rat
WB IHC IF Recommended dilution: Predicted MW: 70kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Background / Function
Displays serine/threonine-specific phosphorylation of myelin basic protein and histone (MBP) in vitro.Soosairajah J, et al. (2005) EMBO J.Ohashi K, et al. (2000) J Biol Chem; 275(5): 3577-82.Edwards DC, et al. (1999) Nat Cell Biol; 1(5): 253-9.
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.