Description
Aliases
PRKMK7; JNKK2; MKK7; MAP2K7; MP2K7
Antibody Type
Polyclonal Antibody
Uniprot ID
Swiss-Prot#: O14733;
NCBI Gene#: 5609;
N
Immunogen
Peptide sequence around phosphorylation site of Serine271(V-D-S(p)-K-A) derived from Human MAP2K7.
Raised In
Rabbit
Species Reactivity
Human Mouse Rat
Tested Applications
WB IHC Recommended dilution: Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Background / Function
The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase specifically activates MAPK8/JNK1 and MAPK9/JNK2, and this kinase itself is phosphorylated and activated by MAP kinase kinase kinases including MAP3K1/MEKK1, MAP3K2/MEKK2,MAP3K3/MEKK5, and MAP4K2/GCK. This kinase is involved in the signal transduction mediating the cell responses to proinflammatory cytokines, and environmental stresses. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found, but only one transcript variant has been supported and defined.Wu Z., Mol. Cell. Biol. 17:7407-7416(1997).Lu X., J. Biol. Chem. 272:24751-24754(1997).Foltz I.N., J. Biol. Chem. 273:9344-9351(1998).
Conjugate
Unconjugated
Storage Buffer
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Modification
Phospho-Ser271
Additional information
| Size | 100?l |
|---|
