M3K1; MAP3K1; MAPKKK1; MEKK1;
NCBI Gene#: 4214;
Peptide sequence around phosphorylation site of threonine 1402(T-G-A(p)-G-F) derived from Human MAP3K1.
Human Mouse Rat
WB IHC Recommended dilution: Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Background / Function
Component of a protein kinase signal transduction cascade. Activates the ERK and JNK kinase pathways by phosphorylation of MAP2K1 and MAP2K4. Activates CHUK and IKBKB, the central protein kinases of the NF-kappa-B pathway.Schmutz J., Nature 431:268-274(2004).Xia Y., Genes Dev. 12:3369-3381(1998).Vinik B.S., Mamm. Genome 6:782-783(1995)
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.