MARCKS(Phospho-Ser162) Antibody

$454.00

Cat. No.: 38818 Categories: ,

Description

Aliases

MACS; MARCS; PKCSL; PRKCSL; Protein kinase C substrate

Antibody Type

Polyclonal Antibody

Uniprot ID

Swiss-Prot: P29966
NCBI Protein: NP_002347.5

Immunogen

Peptide sequence around phosphorylation site of serine 162 (K-K-S(p)-F-K) derived from Human MARCKS.

Raised In

Rabbit

Species Reactivity

Human Mouse Rat

Tested Applications

WB IF Recommended dilution: Predicted MW: 80kd, Western blotting: 1:500~1:1000, Immunofluorescence: 1:100~1:200

Background / Function

MARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein.Pariser H, et al. Proc Natl Acad Sci U S A 2005 Aug 30; 102(35): 12407-12412Nagumo H, et al. Biochem Biophys Res Commun 2001 Jan 26; 280(3): 605-609Yamamoto H, et al. Arch Biochem Biophys 1998 Nov 15; 359(2): 151-159

Conjugate

Unconjugated

Storage Buffer

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form

liquid

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Modification

Phospho-Ser162

Additional information

Size

100?l

Certificate of Analysis