Description
Aliases
MAP kinase kinase 3; MAP2K3; MAPK/ERK kinase 3; MAPKK 3; MEK3
Antibody Type
Polyclonal Antibody
Uniprot ID
Swiss-Prot: P46734
NCBI Protein: NP_002747.2
Immunogen
Peptide sequence around phosphorylation site of serine 189 (V-D-S(p)-V-A) derived from Human MKK3.
Raised In
Rabbit
Species Reactivity
Human Mouse Rat
Tested Applications
WB IHC IF Recommended dilution: Predicted MW: 40kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Background / Function
Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38. Wang W, et al. (2002) Mol Cell Biol ; 22(10): 3389-403. Raingeaud J, et al. (1996) Mol Cell Biol; 16(3): 1247-55.
Conjugate
Unconjugated
Storage Buffer
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Modification
Phospho-Ser189
Additional information
| Size | 100?l |
|---|
