Aliases
Myosin regulatory light polypeptide 9;20 kDa myosin light chain; LC20;MLC-2C;Myosin RLC;Myosin regulatory light chain 2, smooth muscle isoform;Myosin regulatory light chain 9;Myosin regulatory light chain MRLC1;MYL9;MLC2;MRLC1; MYRL2
Antibody Type
Polyclonal Antibody
Uniprot ID
Swiss-Prot#: P24844
NCBI Gene ID: 10398
NC
Immunogen
Peptide sequence around phosphorylation site of tyrosine 118 (A-D-Y(p)-V-R) derived from Human MLC2.
Raised In
Rabbit
Species Reactivity
Human
Tested Applications
WB IF Recommended dilution: Western blotting:1:500~1:1000
Background / Function
Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion. 1) Xia, Y. et al. c-Jun downregulation by HDAC3-dependent transcriptional repression promotes osmotic stress-induced cell apoptosis. Mol. Cell 25, 219–232 (2007).2)Vander Heiden, M. G. et al. Evidence for an alternative glycolytic pathway in rapidly proliferating cells. Science 329, 1492–1499 (2010).3) Fang, D. et al. Phosphorylation of beta-catenin by AKT promotes beta-catenin transcriptional activity. J. Biol. Chem. 282, 11221–11229 (2007).
Conjugate
Unconjugated
Storage Buffer
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Modification
Phospho-Tyr118
