NCBI Protein: NP_002458.2
Peptide sequence around phosphorylation site of threonine 58 (L-P-T(p)-P-P) derived from Human Myc.
Human Mouse Rat
WB IHC Recommended dilution: Predicted MW: 60kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100,
Background / Function
Participates in the regulation of gene transcription. Binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5′-CAC[GA]TG-3′. Seems to activate the transcription of growth-related genes.Jin Z, et al. (2004) J Biol Chem. 279(38): 40209-40219.Welcker M, et al. (2004) Proc Natl Acad Sci U S A. 101(24): 9085-9090.Baudino T A, et al. (2001) Mol Cell Biol. 21: 691-702.Blackwood E M, et al. (1991) Science. 251:1211-1217.Henriksson M, et al. (1996) Adv Cancer Res. 68: 109-182.
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.