Myosin Light Chain 2 (Phospho-Ser19) Antibody

$476.70

Cat. No.: 38769 Categories: ,

Description

Aliases

MLC-2a; MLC2a; MLRA; MYL7; MYLC2A

Antibody Type

Polyclonal Antibody

Uniprot ID

Swiss-Prot: P24844
NCBI Protein: NP_006088.2

Immunogen

Peptide sequence around phosphorylation site of serine 19 (A-T-S(p)-N-V) derived from Human Myosin Light Chain 2.

Raised In

Rabbit

Species Reactivity

Human Mouse Rat

Tested Applications

WB IHC Recommended dilution: Predicted MW: 18kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

Background / Function

Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion Janiak A, et al. (2006) Mol Biol Cell. Apr; 17(4): 1606-1619. Croft DR, et al. (2006) Mol Cell Biol. 2 Jun; 26(12): 4612-4627 Li Z, et al. (2006) Mol Cell Biol. Jun; 26(11): 4240-4256

Conjugate

Unconjugated

Storage Buffer

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form

liquid

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Modification

Phospho-Ser19

Additional information

Size

100?l

Certificate of Analysis