GRIN2B; NMDE2; NME2; NR2B; NR3
NCBI Protein: NP_000825.2
Peptide sequence around phosphorylation site of Tyr1474 (H-V-Y(p)-E-K) derived from Human NMDAR2B.
Human Mouse Rat
WB IF Recommended dilution: Predicted MW: 190kdWestern Blot: 1:500~1000, Immunofluorescence: 1:100~1:200
Background / Function
NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. Babb TL et al.Epilepsy Res. 2005 Mar-Apr;64(1-2):23-30 Chazot PL. Curr Med Chem. 2004 Feb;11(3):389-96 Kakegawa W, et al.Eur J Neurosci. 2003 Feb;17(4):887-91
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.