CD2A1; CDK4I; CDKN2; CDKN2A; CDN2; Cyclin-dependent kinase 4 inhibitor A; cyclin-dependent kinase inhibitor 2A; MTS1; Multiple tumor suppressor 1; p14ARF; p16(INK4a); p16-INK4; P16INK4A
Peptide sequence around phosphorylation site of serine 152 (G-P-S(p)-D-I) derived from Human p16-INK4a.
WB IHC Recommended dilution: Western blotting: 1:500~1:3000, Immunohistochemistry: 1:50~1:100,
Background / Function
Acts as a negative regulator of the proliferation of normal cells by interacting strongly with CDK4 and CDK6. This inhibits their ability to interact with cyclins D and to phosphorylate the retinoblastoma protein.
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.