p27Kip1(Phospho-Thr187) Antibody

$454.00

Cat. No.: 38800 Categories: ,

Description

Aliases

CDKN1B; CDN1B; Cyclin-dependent kinase inhibitor 1B; Cyclin-dependent kinase inhibitor p27; KIP1

Antibody Type

Polyclonal Antibody

Uniprot ID

Swiss-Prot: P46527
NCBI Protein: NP_004055.1

Immunogen

Peptide sequence around phosphorylation site of threonine 187 (E-Q-T(p)-P-K) derived from Human p27Kip1.

Raised In

Rabbit

Species Reactivity

Human Mouse Rat

Tested Applications

WB IHC Recommended dilution: Predicted MW: 27kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100,

Background / Function

Important regulator of cell cycle progression. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Positive regulator of cyclin D-dependent kinases such as CDK4. Regulated by phosphorylation and degradation events.Eguchi H, et al. (2003) Cancer Res; 63(15): 4739-46Le XF, et al. (2003) J Biol Chem;Connor MK, et al. (2003)

Conjugate

Unconjugated

Storage Buffer

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form

liquid

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Modification

Phospho-Thr187

Additional information

Size

100?l

Certificate of Analysis