P38 MAPK(Phospho-Tyr182) Antibody

$454.00

Cat. No.: 39026 Categories: ,

Description

Aliases

MAPK2; MAPKAPK-2; MAPKAPK2

Antibody Type

Polyclonal Antibody

Uniprot ID

Swiss-Prot: Q16539
NCBI Protein: NP _001306.1

Immunogen

Peptide sequence around phosphorylation site of tyrosine 182 (T-G-Y(p)-V-A) derived from Human P38 MAPK.

Raised In

Rabbit

Species Reactivity

Human Mouse Rat

Tested Applications

WB IHC Recommended dilution: Predicted MW: 43kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

Background / Function

Responds to activation by environmental stress, pro-inflammatory cytokines and lipopolysaccharide (LPS) by phosphorylating a number of transcription factors, such as ELK1 and ATF2 and several downstream kinases, such as MAPKAPK2 and MAPKAPK5. Plays a critical role in the production of some cytokines, for example IL-6. May play a role in stabilization of EPO mRNA during hypoxic stress. Isoform Mxi2 activation is stimulated by mitogens and oxidative stress and only poorly phosphorylates ELK1 and ATF2. Isoform Exip may play a role in the early onset of apoptosis. Ming Zheng, et al.(2005) The FASEB Journal. 19: 109-111 Bernt van den et al.(2001) Blink Immunology, 166: 582-587 Arshad Rahman, et al. (2004) Am J Physiol Lung Cell Mol Physiol 287: L1017-L1024 Osamu Yoshino, et al. (2003) Endocrinology & Metabolism Vol. 88: 2236-2241

Conjugate

Unconjugated

Storage Buffer

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form

liquid

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Modification

Phospho-Tyr182

Additional information

Size

100?l

Certificate of Analysis