p44/42 MAP Kinase(Phospho-Thr202) Antibody

$476.70

Cat. No.: 39020 Categories: ,

Description

Aliases

ERK; ERT2; Extracellular signal- regulated kinase 1; Insulin-stimulated MAP2 kinase; MAP kinase 1

Antibody Type

Polyclonal Antibody

Uniprot ID

Swiss-Prot: P27361
NCBI Protein: NP_001035145.

Immunogen

Peptide sequence around phosphorylation site of threonine 202 (F-L-T(p)-E-Y) derived from Human p44/42 MAP Kinase.

Raised In

Rabbit

Species Reactivity

Human Mouse Rat

Tested Applications

WB IHC IF Recommended dilution: Predicted MW: 42 44 kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

Background / Function

Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 TETE HANNKEN, et al. (2000) Am Soc Nephrol 11:1387-1397 Omar D. PerezNature et al. (2002) Biotechnology 20: 155 – 162 Jingui Yu, et al. (2005) Anesth Analg 101: 315-321 Hironobu Ihn et al.(2000) Immunology 165: 2149-2155

Conjugate

Unconjugated

Storage Buffer

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form

liquid

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Modification

Phospho-Thr202

Additional information

Size

100μl

Certificate of Analysis