Description
Aliases
NCF1; P47 phox; NCF-47K;
Antibody Type
Polyclonal Antibody
Uniprot ID
Swiss-Prot#: P14598;
NCBI Gene#: 653361;
Immunogen
Peptide sequence around phosphorylation site of Serine 345(P-Q-S(p)-P-G) derived from Human Nuclear p47 phox.
Raised In
Rabbit
Species Reactivity
Human
Tested Applications
WB IHC Recommended dilution: Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Background / Function
NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).Volpp B.D., Proc. Natl. Acad. Sci. U.S.A. 86:7195-7199(1989).Lomax K.J., Science 245:409-412(1989).Rodaway A.R.F., Mol. Cell. Biol. 10:5388-5396(1990).
Conjugate
Unconjugated
Storage Buffer
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Modification
Phospho-Ser345
Additional information
| Size | 100?l |
|---|
