p53(Phospho-Ser15) Antibody

$476.70

Cat. No.: 39086 Categories: ,

Description

Aliases

Tumor suppressor p53; Phosphoprotein p53; Antigen NY-CO-13; TP53;

Antibody Type

Polyclonal Antibody

Uniprot ID

Swiss-Prot: P04637
NCBI Protein: NP_000537.3

Immunogen

Peptide sequence around phosphorylation site of serine 15 (P-L-S(p)-Q-E) derived from Human p53.

Raised In

Rabbit

Species Reactivity

Human

Tested Applications

WB IHC IF Recommended dilution: Predicted MW: 53kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

Background / Function

Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. Implicated in Notch signaling cross-over. Lin T, et al. (2005) Nat Cell Biol; 7(2): 165-71. Vega FM, et al. (2004) Mol Cell Biol; 24(23): 10366-80. Li J, et al. (2004) J Biol Chem; 279(40): 41275-9. Wang J, et al. (2004) J Biol Chem; 279(38): 39584-92.

Conjugate

Unconjugated

Storage Buffer

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form

liquid

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Modification

Phospho-Ser15

Additional information

Size

100?l

Certificate of Analysis