Description
Aliases
Tumor suppressor p53; Phosphoprotein p53; Antigen NY-CO-13; TP53;
Antibody Type
Polyclonal Antibody
Uniprot ID
Swiss-Prot: P04637
NCBI Protein: NP_000537.3
Immunogen
Peptide sequence around phosphorylation site of serine 315 (S-S-S(p)-P-Q) derived from Human p53.
Raised In
Rabbit
Species Reactivity
Human
Tested Applications
WB IHC IF Recommended dilution: Predicted MW: 53kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Background / Function
Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. Implicated in Notch signaling cross-over.Lu, H. et al. (1997) Mol. Cell. Biol. 17, 5923-5934.Lohrum, M. et.al. (1996) Oncogene 13, 2527-2539.Posp
Conjugate
Unconjugated
Storage Buffer
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Modification
Phospho-Ser315
Additional information
| Size | 100?l |
|---|
