Antigen NY-CO-13; Phosphoprotein p53; TP53; Tumor suppressor p53;
NCBI Protein: NP_000537.3
Peptide sequence around phosphorylation site of serine 6 (P-Q-S(p)-D-P) derived from Human p53.
WB IHC Recommended dilution: Predicted MW: 53kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100,
Background / Function
Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. Implicated in Notch signaling cross-over.Lin T, et al. (2005) Nat Cell Biol; 7(2): 165-71.Vega FM, et al. (2004) Mol Cell Biol; 24(23): 10366-80.Li J, et al. (2004) J Biol Chem; 279(40): 41275-9.Wang J, et al. (2004) J Biol Chem; 279(38): 39584-92.
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.