Aliases
KS6B1; P70-S6K; RPS6KB1; S6K;
Antibody Type
Polyclonal Antibody
Uniprot ID
Swiss-Prot: P23443
NCBI Protein: NP_003152.1
Immunogen
Peptide sequence around phosphorylation site of serine 411 (I-R-S(p)-P-R) derived from Human p70 S6 Kinase.
Raised In
Rabbit
Species Reactivity
Human Mouse Rat
Tested Applications
WB IHC IF Recommended dilution: Predicted MW: 70 85 kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Background / Function
Phosphorylates specifically ribosomal protein S6 in response to insulin or several classes of mitogens. Promotes protein synthesis by phosphorylating PDCD4 at ‘Ser-67’ and targeting it for degradation.Satoru Eguchi et al. (1999) J Biol Chem, Vol. 274: 36843-36851Papst PJ, et al. (1998) J Biol Chem. 273(24):15077-84.Ulrike Krause et al. (2002) Eur. J. Biochem. 269: 3751-3759 cLe, X.F, et al. (2003) Oncogene 22: 484
Conjugate
Unconjugated
Storage Buffer
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Modification
Phospho-Ser411
