Description
Aliases
p21-activated kinase 1; PAK-1; p65-PAK; Alpha-PAK;
Antibody Type
Polyclonal Antibody
Uniprot ID
Swiss-Prot: Q13153
NCBI Protein: NP_001122092.
Immunogen
Peptide sequence around phosphorylation site of threonine 212 (P-V-T(p)-P-T) derived from Human PAK1.
Raised In
Rabbit
Species Reactivity
Human Mouse Rat
Tested Applications
WB IHC IF Recommended dilution: Predicted MW: 68kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Background / Function
The activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Involved in regulation of microtubule biogenesis through phosphorylation of TBCB. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2.Alexander K, et al. (2004) Mol Cell Biol; 24: 2808-2819Thiel DA, et al. (2002) Curr Biol; 12:1227-1232Rashid T, et al. (2001) J. Biol. Chem; 276: 49043 – 49052.
Conjugate
Unconjugated
Storage Buffer
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Modification
Phospho-Thr212
Additional information
| Size | 100?l |
|---|
