NCBI Protein: NP_001074324.
Peptide sequence around phosphorylation site of tyrosine 118 (H-V-Y(p)-S-F) derived from Human Paxillin.
Human Mouse Rat
WB IHC Recommended dilution: Predicted MW: 68kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100,
Background / Function
Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion).Tao WA, et al.(2005)Nat Methods.2(8): 591-598.Zhang Y, et al.(2005) Mol Cell Proteomics.4(9): 1240-1250.Sanders MA, et al.(2005)J Biol Chem.280(25): 23516-23522.Rush J, et al.(2004) Nat Biotechnol. 23(1): 94-101.
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.