NCBI Protein: NP_001074324.
Peptide sequence around phosphorylation site of tyrosine 88 (P-V-Y(p)-G-S) derived from Human Paxillin.
WB IF Recommended dilution: Predicted MW: 68kd, Western blotting: 1:500~1:1000, Immunofluorescence: 1:100~1:200
Background / Function
An antiphosphotyrosine antibody was used to identify proteins that are phosphorylated in Rous sarcoma virus-transformed chick embryo fibroblasts, and a 76-kD protein was obtained that localizes to focal adhesions at the ends of actin-containing stress fibers in nontransformed cells (Ref.1). This protein was purified from chicken gizzard smooth muscle, and was named Pxn (Paxillin) (‘paxillus’ means ‘small stake’ or ‘peg’ in Latin) as a protein tethered to the membrane at focal adhesions Turner, C.E. (2000) J. Cell Sci. 113, 4139-4140Burridge, K. et al. (1992) J. Cell Biol. 119, 893-903.Bellis, S. L. et al. (1997) Biochem. J. 325, 375-381.
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.