PDPK1; PkB kinase; Protein kinase B kinase; hPDK1; kinase PDK1
NCBI Protein: NP_002604.1
Peptide sequence around phosphorylation site of serine 241 (A-N-S(p)-F-V) derived from Human PDK1.
Human Mouse Rat
WB IHC IF Recommended dilution: Predicted MW: 63kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Background / Function
Phosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development.Scheid MP,et al. (2005)Mol Cell Biol; 25(6): 2347-63Chen H, et al. (2001) Biochemistry; 40(39): 11851-9Sato S,et al. (2002) J Biol Chem; 277(42): 39360-7Lim MA, et al.(2003)Proc Natl Acad Sci U S A; 100(24): 14006-11
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.