Description
Aliases
KPCD; PKC-delta; PRKCD; kinase PKC-delta; nPKC-delta
Antibody Type
Polyclonal Antibody
Uniprot ID
Swiss-Prot: Q05655
NCBI Protein: NP_006245.2
Immunogen
Peptide sequence around phosphorylation site of serine 645 (R-L-S(p)-Y-S) derived from Human PKCd.
Raised In
Rabbit
Species Reactivity
Human Mouse Rat
Tested Applications
WB IHC Recommended dilution: Predicted MW: 78kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100,
Background / Function
This is calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. May play a role in antigen-dependent control of B-cell function. Phosphorylates MUC1 in the C-terminal and regulates the interaction between MUC1 and beta-catenin. Kei Sakamoto, et,al. (2003) Am J Physiol Endocrinol Metab ; 285: E1081 – E1088. Ling Zhang, et,al. (2004) J. Biol. Chem ; 279: 28315 – 28319. Kristof Van Kolen et,al. (2006) FEBS J ; 273: 1843 – 1854. Martin Villalba, et,al. (2002) J. Cell Biol ; 157: 253.
Conjugate
Unconjugated
Storage Buffer
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Modification
Phospho-Ser645
Additional information
| Size | 100?l |
|---|
