PKM2(phospho-Ser37) Antibody

$476.70

Cat. No.: 38870 Categories: ,

Description

Aliases

PKM, PK3, OIP3, PK2

Antibody Type

Polyclonal Antibody

Uniprot ID

Swiss-Prot#: P14618 NCBI Protein#: NP_872270.1

Immunogen

Peptide sequence around phosphorylation site of serine 37(I-D-S(p)-P-P) derived from Human PKM2.

Raised In

Rabbit

Species Reactivity

Human

Tested Applications

WB IF Recommended dilution: Predicted MW: 60kdWestern Blot: 1:500~1000 

Background / Function

Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.Weiwei Yang1, Yanhua Zheng1, Yan Xia1, Haitao Ji, "ERK1/2-dependent phosphorylation and nucleartranslocation of PKM2 promotes the Warburg effect." Nature Cell Biology(2012)|doi:10.1038/ncb2629Received16 August 2012 Accepted24 October 2012 Published online25 November 2012

Conjugate

Unconjugated

Storage Buffer

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form

liquid

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Modification

Phospho-Ser37

Additional information

Size

100?l

Certificate of Analysis