Description
Aliases
ADRB2; E2AK2; EIF2AK2; EIF2aK; PRKR
Antibody Type
Polyclonal Antibody
Uniprot ID
Swiss-Prot: P19525
NCBI Protein: NP_001129123
Immunogen
Peptide sequence around phosphorylation site of threonine 446 (K-R-T(p)-R-S) derived from Human PKR.
Raised In
Rabbit
Species Reactivity
Human Mouse
Tested Applications
WB IHC IF Recommended dilution: Predicted MW: 68kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Background / Function
Following activation by double-stranded RNA in the presence of ATP, the kinase becomes autophosphorylated and can catalyze the phosphorylation of the translation initiation factor EIF2S1, which leads to an inhibition of the initiation of protein synthesis. Double-stranded RNA is generated during the course of a viral infection. Abujiang Pataer, et,al. (2002) Cancer Res; 62: 2239. K. D. Ryman, et,al. (2005) J. Virol; 79: 1487 – 1499. Susana Guerra, et,al. (2006) J. Biol. Chem; 281: 18734 – 18745.
Conjugate
Unconjugated
Storage Buffer
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Modification
Phospho-Thr446
Additional information
| Size | 100?l |
|---|
