PKR(Phospho-Thr446) Antibody

$454.00

Cat. No.: 39033 Categories: ,

Description

Aliases

ADRB2; E2AK2; EIF2AK2; EIF2aK; PRKR

Antibody Type

Polyclonal Antibody

Uniprot ID

Swiss-Prot: P19525
NCBI Protein: NP_001129123

Immunogen

Peptide sequence around phosphorylation site of threonine 446 (K-R-T(p)-R-S) derived from Human PKR.

Raised In

Rabbit

Species Reactivity

Human Mouse

Tested Applications

WB IHC IF Recommended dilution: Predicted MW: 68kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

Background / Function

Following activation by double-stranded RNA in the presence of ATP, the kinase becomes autophosphorylated and can catalyze the phosphorylation of the translation initiation factor EIF2S1, which leads to an inhibition of the initiation of protein synthesis. Double-stranded RNA is generated during the course of a viral infection. Abujiang Pataer, et,al. (2002) Cancer Res; 62: 2239. K. D. Ryman, et,al. (2005) J. Virol; 79: 1487 – 1499. Susana Guerra, et,al. (2006) J. Biol. Chem; 281: 18734 – 18745.

Conjugate

Unconjugated

Storage Buffer

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form

liquid

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Modification

Phospho-Thr446

Additional information

Size

100?l

Certificate of Analysis