Phosphoinositide phospholipase C; Phospholipase C-gamma-1;
NCBI Protein: NP_002651.2
Peptide sequence around phosphorylation site of tyrosine 771 (P-D-Y(p)-G-A) derived from Human PLC-g1.
Human Mouse Rat
WB IF Recommended dilution: Predicted MW: 155kd, Western blotting: 1:500~1:1000, Immunofluorescence: 1:100~1:200
Background / Function
PLC-gamma is a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase.Yue, C. et al. (1998) J. Biol. Chem. 273, 18023-18027. Margolis, B. et al. (1989) Cell 57, 1101-1107. Yue, C. et al. (2000) J. Biol. Chem. 275, 30220-30225.
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.