Cytolysin, Lymphocyte pore-forming protein.PRF1, PFP
Recombinant human Perforin-1 protein (22-555AA)
ELISA;Not yet tested in other applications.
Background / Function
Plays a key role in secretory granule-dependent cell death, and in defense against virus-infected or neoplastic cells. Plays an important role in killing other cells that are recognized as non-self by the immune system, e.g. in transplant rejection or some forms of autoimmune disease. Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores. Promotes cytolysis and apoptosis of target cells by facilitating the uptake of cytotoxic granzymes.
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Caprylic Acid Ammonium Sulfate Precipitation purified
Literature “Structure and function of human perforin.”Lichtenheld M.G., Olsen K.J., Lu P., Lowrey D.M., Hameed A., Hengartner H., Podack E.R.Nature 335:448-451(1988)  “Molecular cloning and chromosomal assignment of a human perforin (PFP) gene.”Shinkai Y.,