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Protease Inhibitor Cocktail [100X], General-EDTA Free

$76.00$117.00

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Cat. No.: 10471 Categories: , ,

Description

General Description

Our General Protease Inhibitor Cocktail contains AEBSF, Aprotinin, Bestatin, E64, Leupeptin and other proprietary component(s) at 100X concentration, optimized for inhibiting broad spectrum of proteases present in the protein extract samples and is EDTA Free.

EDTA-free Protease Inhibitor Cocktail, inhibits a broad spectrum of serine and cysteine proteases. In contrast to other complete tablets they do not contain EDTA, leaving the stability and the function of metal-dependant proteins unaffected. The affinity purification of Poly-His-tagged fusion proteins using IMAC (Immobilized Metal Affinity Chromatography) is also facilitated (no dialysis necessary).

Due to the optimized composition of the solution mix, they show excellent inhibition of serine and cysteine proteases, and are well suited for the protection of proteins isolated from animal tissues, plants, yeast, and bacteria.

 

Application

EDTA-free Protease Inhibitor Cocktail has been used as a component of radioimmunoprecipitation assay (RIPA) lysis buffer for resuspending Chinese hamster ovary (CHO) cells. It has also been used as a component of resuspension buffer for HCT116 colorectal cancer cells.

EDTA-free Protease Inhibitor Cocktail has been used as as a component of:
• immunoprecipitation lysis buffer (IPLS) for lysis of human embryonic kidney cells 293 (HEK293T cells) for glutathione co-precipitation
• radioimmunoprecipitation assay (RIPA) buffer for lysis of cells for western blotting
• buffer for the lysis of bacterial cells

 

Additional information

Size

1 ml, 2 ml

Certificate of Analysis