Description
Aliases
FADK 2; FAK2; Focal adhesion kinase 2; PTK2B; Proline-rich tyrosine kinase 2 RAFTK
Antibody Type
Polyclonal Antibody
Uniprot ID
Swiss-Prot: Q14289
NCBI Protein: NP_004094.3
Immunogen
Peptide sequence around phosphorylation site of tyrosine 402 (D-I-Y(p)-A-E) derived from Human Pyk2.
Raised In
Rabbit
Species Reactivity
Human Mouse Rat
Tested Applications
WB IHC IF Recommended dilution: Predicted MW: 116kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Background / Function
Involved in calcium induced regulation of ion channel and activation of the map kinase signaling pathway. May represent an important signaling intermediate between neuropeptide activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. Interacts with the SH2 domain of Grb2. May phosphorylate the voltage-gated potassium channel protein Kv1.2. Its activation is highly correlated with the stimulation of c-Jun N-terminal kinase activity. Involved in osmotic stress-dependent SNCA ‘Tyr-125’ phosphorylation.Gluck SL, et al. (2004) J Clin Invest; 114(12): 1696-1699Benzing T, et al. (2001) Proc Natl Acad Sci U S A; 98(17): 9784-9789Tian D, et al. (2002) Mol Cell Biol; 22(8): 2650-2662Lu Z, et al. (2001) Mol Cell Biol; 21(12): 4016-4031Krishnan HH, et al. (2006) J Virol; 80(3): 1167-1180
Conjugate
Unconjugated
Storage Buffer
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Modification
Phospho-Tyr402
Additional information
| Size | 100μl |
|---|
