Pyk2(Phospho-Tyr402) Antibody

$454.00

Cat. No.: 39016 Categories: ,

Description

Aliases

FADK 2; FAK2; Focal adhesion kinase 2; PTK2B; Proline-rich tyrosine kinase 2 RAFTK

Antibody Type

Polyclonal Antibody

Uniprot ID

Swiss-Prot: Q14289
NCBI Protein: NP_004094.3

Immunogen

Peptide sequence around phosphorylation site of tyrosine 402 (D-I-Y(p)-A-E) derived from Human Pyk2.

Raised In

Rabbit

Species Reactivity

Human Mouse Rat

Tested Applications

WB IHC IF Recommended dilution: Predicted MW: 116kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

Background / Function

Involved in calcium induced regulation of ion channel and activation of the map kinase signaling pathway. May represent an important signaling intermediate between neuropeptide activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. Interacts with the SH2 domain of Grb2. May phosphorylate the voltage-gated potassium channel protein Kv1.2. Its activation is highly correlated with the stimulation of c-Jun N-terminal kinase activity. Involved in osmotic stress-dependent SNCA ‘Tyr-125’ phosphorylation.Gluck SL, et al. (2004) J Clin Invest; 114(12): 1696-1699Benzing T, et al. (2001) Proc Natl Acad Sci U S A; 98(17): 9784-9789Tian D, et al. (2002) Mol Cell Biol; 22(8): 2650-2662Lu Z, et al. (2001) Mol Cell Biol; 21(12): 4016-4031Krishnan HH, et al. (2006) J Virol; 80(3): 1167-1180

Conjugate

Unconjugated

Storage Buffer

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form

liquid

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Modification

Phospho-Tyr402

Additional information

Size

100μl

Certificate of Analysis