Description
Product name: Rabbit α-Goat IgG, Horseradish peroxidase (HRP) labeled
Host species: Rabbit
Target species: Goat
Specificity: By immunoelectrophoresis and ELISA this antibody reacts specifically with Goat IgG and with light chains common to other Goat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.
Tested applications: Suitable for Western Blot, ELISA, ICC, IHC-P
Conjugation: HRP
Form: Liquid
Storage: Shipped at 4°C, Store at +4°C upon receiving
Storage buffer: 0.2% BSA, PBS
Preservative: 0.1% Proclin
Concentration: 1 mg/ml
Purity: Immunogen affinity purified, using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).
Conjugation notes: Molar enzyme & antibody protein ratio is 4:1
Clonality: Polyclonal
Isotype: IgG
Recommended Dilutions: Below are the recommended starting dilutions (optimal dilutions/concentrations) and should be determined by the end user:
|
Application |
Recommended Dilution |
|
Western Blot |
1:2000 – 1:20000 |
|
1:5000 – 1:30000 |
|
|
ELISA |
1:10000 – 1:100000 |
Fig: Western blot – Probed with Rabbit α-Goat IgG, Horseradish peroxidase (HRP) labeled
All lanes: Anti-beta Actin antibody (1 µg/ml) and HeLa: Human epithelial carcinoma cell line, Whole Cell Lysate at 20 µg per lane
Secondary
Lanes 1 – 2: Rabbit α-Goat IgG, Horseradish peroxidase (HRP) labeled at 1:5000 dilution
Lanes 3 – 4: Rabbit α-Goat IgG, Horseradish peroxidase (HRP) labeled at 1:10000 dilution
Lanes 5 – 6: Rabbit α-Goat IgG, Horseradish peroxidase (HRP) labeled at 1:20000 dilution
Developed using the ECL technique
Exposure time: 5 minutes
Additional information
| Size | 1.0ml |
|---|
