Rabbit a-Goat IgG, Horseradish peroxidase (HRP) labeled(1mg/ml)

$353.85

Description

Product name: Rabbit α-Goat IgG, Horseradish peroxidase (HRP) labeled

Host species: Rabbit

Target species: Goat

Specificity: By immunoelectrophoresis and ELISA this antibody reacts specifically with Goat IgG and with light chains common to other Goat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.

Tested applications: Suitable for Western Blot, ELISA, ICC, IHC-P

Conjugation: HRP

Form: Liquid

Storage: Shipped at 4°C, Store at +4°C upon receiving

Storage buffer: 0.2% BSA, PBS

Preservative: 0.1% Proclin

Concentration: 1 mg/ml 

Purity: Immunogen affinity purified, using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).

Conjugation notes: Molar enzyme & antibody protein ratio is 4:1

Clonality: Polyclonal

Isotype: IgG

Recommended Dilutions: Below are the recommended starting dilutions (optimal dilutions/concentrations) and should be determined by the end user:

 Application

Recommended Dilution

Western Blot

1:2000 – 1:20000
Chemiluminescent detection

1:5000 – 1:30000
Colorimetric detection

ELISA

1:10000 – 1:100000

Fig: Western blot – Probed with Rabbit α-Goat IgG, Horseradish peroxidase (HRP) labeled

All lanes: Anti-beta Actin antibody (1 µg/ml) and HeLa: Human epithelial carcinoma cell line, Whole Cell Lysate at 20 µg per lane 

Secondary

Lanes 1 – 2: Rabbit α-Goat IgG, Horseradish peroxidase (HRP) labeled at 1:5000 dilution

Lanes 3 – 4: Rabbit α-Goat IgG, Horseradish peroxidase (HRP) labeled at 1:10000 dilution

Lanes 5 – 6: Rabbit α-Goat IgG, Horseradish peroxidase (HRP) labeled at 1:20000 dilution

Developed using the ECL technique

Exposure time: 5 minutes

Additional information

Size

1.0ml

Certificate of Analysis