RIPK2 Antibody, HRP conjugated

$210.00$377.00

Description

Aliases

CARD-containing interleukin-1 beta-converting enzyme-associated kinase RIP-like-interacting CLARP kinase Receptor-interacting protein 2 Tyrosine-protein kinase RIPK2 EC=2.7.10.2 RIPK2 CARDIAK, RICK, RIP2 UNQ277/PRO314/PRO34092

Antibody Type

Polyclonal Antibody

Species

Human

Uniprot ID

O43353

Immunogen

Recombinant human Receptor-interacting serine/threo nine-protein kinase 2 protein (1-540AA)

Raised In

Rabbit

Species Reactivity

Human

Tested Applications

ELISA;Not yet tested in other applications.

Background / Function

Serine/threonine/tyrosine kinase that plays an essential role in modulation of innate and adaptive immune responses. Upon stimulation by bacterial peptidoglycans, NOD1 and NOD2 are activated, oligomerize and recruit RIPK2 through CARD-CARD domains. Contributes to the tyrosine phosphorylation of the guanine exchange factor ARHGEF2 through Src tyrosine kinase leading to NF-kappaB activation by NOD2. Once recruited, RIPK2 autophosphorylates and undergoes ‘Lys-63’-linked polyubiquitination by E3 ubiquitin ligases XIAP, BIRC2 and BIRC3. The polyubiquitinated protein mediates the recruitment of MAP3K7/TAK1 to IKBKG/NEMO and induces ‘Lys-63’-linked polyubiquitination of IKBKG/NEMO and subsequent activation of IKBKB/IKKB. In turn, NF-kappa-B is released from NF-kappa-B inhibitors and translocates into the nucleus where it activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. Plays also a role during engagement of the T-cell receptor (TCR) in promoting BCL10 phosphorylation and subsequent NF-kappa-B activation.

Isotype

IgG

Conjugate

HRP

Storage Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.

Purity

Caprylic Acid Ammonium Sulfate Precipitation purified

Research

Immunology

Literature

[1]RICK, a novel protein kinase containing a caspase recruitment domain, interacts with CLARP and regulates CD95-mediated apoptosis.Inohara N., del Peso L., Koseki T., Chen S., Nunez G.J. Biol. Chem. 273:12296-12300(1998) [2]RIP2 is a novel NF-kappaB-ac

Additional information

Size

50μg, 100μg

Certificate of Analysis