Description
Aliases
JNKK; JNKK1; MAP2K4; MAPK/ERK kinase 4; MAPKK 4
Antibody Type
Polyclonal Antibody
Uniprot ID
Swiss-Prot: P45985
NCBI Protein: NP_003001.1
Immunogen
Peptide sequence around phosphorylation site of threonine261 (A-K-T(p)-RD) derived from Human SEK1/MKK4.
Raised In
Rabbit
Species Reactivity
Human Mouse Rat
Tested Applications
IHC IF Recommended dilution: Predicted MW: 44kd, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Background / Function
Dual specificity kinase that activates the JUN kinases MAPK8 (JNK1) and MAPK9 (JNK2) as well as MAPK14 (p38) but not MAPK1 (ERK2) or MAPK3 (ERK1).Wang W, et al. (2002) Mol Cell Biol; 22(10): 3389-403.Leung IW, et al. (2001) J Biol Chem; 276(3): 1961-7.
Conjugate
Unconjugated
Storage Buffer
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Modification
Phospho-Thr261
Additional information
| Size | 100?l |
|---|
