JNKK; JNKK1; MAP2K4; MAPK/ERK kinase 4; MAPKK 4
NCBI Protein: NP_003001.1
Peptide sequence around phosphorylation site of threonine261 (A-K-T(p)-RD) derived from Human SEK1/MKK4.
Human Mouse Rat
IHC IF Recommended dilution: Predicted MW: 44kd, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Background / Function
Dual specificity kinase that activates the JUN kinases MAPK8 (JNK1) and MAPK9 (JNK2) as well as MAPK14 (p38) but not MAPK1 (ERK2) or MAPK3 (ERK1).Wang W, et al. (2002) Mol Cell Biol; 22(10): 3389-403.Leung IW, et al. (2001) J Biol Chem; 276(3): 1961-7.
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.