BBP; CW17; SF01; ZFM1; ZNF162
NCBI Gene#: 7536;
Peptide sequence around phosphorylation site of Serine 82(S-P-S(p)-P-E) derived from Human SF1.
WB IHC Recommended dilution: Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Background / Function
Necessary for the ATP-dependent first step of spliceosome assembly. Binds to the intron branch point sequence (BPS) 5′-UACUAAC-3′ of the pre-mRNA. May act as transcription repressor. Susan Y. Park, Development, May 2005; 132: 2415 – 2423. Dujin Zhou, Mol. Endocrinol., Jul 2000; 14: 986. Dagmar Wilhelm, Genes & Dev., Jul 2002; 16: 1839. Philipp Gut,Development, Oct 2005; 132: 4611 – 4619.
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.