Description
Aliases
BBP; CW17; SF01; ZFM1; ZNF162
Antibody Type
Polyclonal Antibody
Uniprot ID
Swiss-Prot#: Q15637;
NCBI Gene#: 7536;
N
Immunogen
Peptide sequence around phosphorylation site of Serine 82(S-P-S(p)-P-E) derived from Human SF1.
Raised In
Rabbit
Species Reactivity
Human Mouse
Tested Applications
WB IHC Recommended dilution: Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Background / Function
Necessary for the ATP-dependent first step of spliceosome assembly. Binds to the intron branch point sequence (BPS) 5′-UACUAAC-3′ of the pre-mRNA. May act as transcription repressor. Susan Y. Park, Development, May 2005; 132: 2415 – 2423. Dujin Zhou, Mol. Endocrinol., Jul 2000; 14: 986. Dagmar Wilhelm, Genes & Dev., Jul 2002; 16: 1839. Philipp Gut,Development, Oct 2005; 132: 4611 – 4619.
Conjugate
Unconjugated
Storage Buffer
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Modification
Phospho-Ser82
Additional information
| Size | 100?l |
|---|
