Description
Aliases
Spleen tyrosine kinase
Antibody Type
Polyclonal Antibody
Uniprot ID
Swiss-Prot: P43405
NCBI Protein: NP_001128524.
Immunogen
Peptide sequence around phosphorylation site of tyrosine 323 (N-P-Y(p)-E- P) derived from Human syk.
Raised In
Rabbit
Species Reactivity
Human
Tested Applications
WB IHC IF Recommended dilution: Predicted MW: 72kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Background / Function
Positive effector of BCR-stimulated responses. Couples the B-cell antigen receptor (BCR) to the mobilization of calcium ion either through a phosphoinositide 3-kinase-dependent pathway, when not phosphorylated on tyrosines of the linker region, or through a phospholipase C-gamma-dependent pathway, when phosphorylated on Tyr-348 and Tyr-352. Thus the differential phosphorylation of Syk can determine the pathway by which BCR is coupled to the regulation of intracellular calcium ion Zhang, J. et al. (2000) J. Biol. Chem. 275, 35442-35447.Turner, M. et al. (2000) Immunol. Today 21, 148-154.Decker, M. et al. (1998) J. Biol. Chem. 273, 8867-8874.Law, C.L. et al. (1996) Mol. Cell. Biol. 16, 1305-1315.
Conjugate
Unconjugated
Storage Buffer
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Modification
Phospho-Tyr323
Additional information
Size | 100μl |
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