syk(phospho-Tyr323) Antibody

$476.70

Cat. No.: 39047 Categories: ,

Description

Aliases

Spleen tyrosine kinase

Antibody Type

Polyclonal Antibody

Uniprot ID

Swiss-Prot: P43405
NCBI Protein: NP_001128524.

Immunogen

Peptide sequence around phosphorylation site of tyrosine 323 (N-P-Y(p)-E- P) derived from Human syk.

Raised In

Rabbit

Species Reactivity

Human

Tested Applications

WB IHC IF Recommended dilution: Predicted MW: 72kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

Background / Function

Positive effector of BCR-stimulated responses. Couples the B-cell antigen receptor (BCR) to the mobilization of calcium ion either through a phosphoinositide 3-kinase-dependent pathway, when not phosphorylated on tyrosines of the linker region, or through a phospholipase C-gamma-dependent pathway, when phosphorylated on Tyr-348 and Tyr-352. Thus the differential phosphorylation of Syk can determine the pathway by which BCR is coupled to the regulation of intracellular calcium ion Zhang, J. et al. (2000) J. Biol. Chem. 275, 35442-35447.Turner, M. et al. (2000) Immunol. Today 21, 148-154.Decker, M. et al. (1998) J. Biol. Chem. 273, 8867-8874.Law, C.L. et al. (1996) Mol. Cell. Biol. 16, 1305-1315.

Conjugate

Unconjugated

Storage Buffer

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form

liquid

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Modification

Phospho-Tyr323

Additional information

Size

100μl

Certificate of Analysis