MAPT; MTAPT; MTBT1; Neurofibrillary tangle protein; PHF-tau
NCBI Protein: NP _001116538
Peptide sequence around phosphorylation site of threonine 205 (P-G-T(p)-P-G) derived from Human Tau.
Human Mouse Rat
WB IHC Recommended dilution: Predicted MW: 48 62 78 kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100,
Background / Function
Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.del C Alonso A, et al. (2004) J Biol Chem; 279(33): 34873-81Li G, Yin H, Kuret J (2004) J Biol Chem; 279(16): 15938-45Giasson BI, et al. (2002) Biochemistry; 41(51): 15376-87Liu F, et al. (2002) FEBS Lett; 530(1-3): 209Taniguchi T, et al. (2001) J Biol Chem; 276(13): 10025-31
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.