Tau(Phospho-Thr231) Antibody

$454.00

Cat. No.: 38767 Categories: ,

Description

Aliases

MAPT; MTAPT; MTBT1; Neurofibrillary tangle protein; PHF-tau

Antibody Type

Polyclonal Antibody

Uniprot ID

Swiss-Prot: P10636
NCBI Protein: NP _001116538

Immunogen

Peptide sequence around phosphorylation site of threonine 231 (V-R-T(p)-P-P) derived from Human Tau.

Raised In

Rabbit

Species Reactivity

Human Mouse Rat

Tested Applications

WB IHC Recommended dilution: Predicted MW: 48 62 78 kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100,

Background / Function

Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.Yen S.H.et.al. (1997) Neurochem. 69:1709-1719.Beausoleil S.A.et.al. (2004) Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135.Wintjens R.et.al. (2001) Biol. Chem. 276:25150-25156.Babu J.R.et.al.(2005) Neurochem. 94:192-203.Goedert M., Spillantini M.G. (2000) Biochim. Biophys. Acta 1502:110-121.

Conjugate

Unconjugated

Storage Buffer

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form

liquid

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Modification

Phospho-Thr231

Additional information

Size

100?l

Certificate of Analysis