Triton® X-114


Cat. No.: 10340 Category:


General description

Triton X-114 is a non-ionic detergent utilized for solubilizing membranes having reversible phase-separation property, useful for the separation of intrinsic membrane proteins. This octylphenol ethoxylate is compatible with anionic, cationic, and other non-ionic surfactants and chemically stable in most acidic & alkaline solutions. Triton X-114 solution is homogeneous at 0°C but phase separation into aqueous phase and a detergent phase occurs above 20°C. Phase separation of proteins in solutions of Triton X-114 has been investigated.


Synonym:                            Polyethylene glycol tert-octylphenyl ether

CAS No.:                              9036-19-5

Molecular Formula:           C14H22O•[C2H4O]7-8 for n =8

Absorbance (254nm):       0.18 (0.05% w/v)

Cloud Point:                        23°C

InChI Key:                            HNLXNOZHXNSSPN-UHFFFAOYSA-N

Molecular Weight:             537

Composition:                      t-Oct-C6H4-(OCH2CH2) xOH, x = 7-8

Refractive index:                n20/D 1.488 (lit.)

CMC:                                    0.2 mM (20-25°C)

Density:                               1.058 g/mL at 25°C (lit.)



  • to remove endotoxin from solutions of protein using phase separation technique
  • in the extraction and purification of DNA from endotoxin
  • in the biphasic extraction of lipoproteins from bacterial cell wall

Additional information


500 ml, 1 L

Certificate of Analysis