Description
General description
Triton X-114 is a non-ionic detergent utilized for solubilizing membranes having reversible phase-separation property, useful for the separation of intrinsic membrane proteins. This octylphenol ethoxylate is compatible with anionic, cationic, and other non-ionic surfactants and chemically stable in most acidic & alkaline solutions. Triton X-114 solution is homogeneous at 0°C but phase separation into aqueous phase and a detergent phase occurs above 20°C. Phase separation of proteins in solutions of Triton X-114 has been investigated.
Synonym: Polyethylene glycol tert-octylphenyl ether
CAS No.: 9036-19-5
Molecular Formula: C14H22O•[C2H4O]7-8 for n =8
Absorbance (254nm): 0.18 (0.05% w/v)
Cloud Point: 23°C
InChI Key: HNLXNOZHXNSSPN-UHFFFAOYSA-N
Molecular Weight: 537
Composition: t-Oct-C6H4-(OCH2CH2) xOH, x = 7-8
Refractive index: n20/D 1.488 (lit.)
CMC: 0.2 mM (20-25°C)
Density: 1.058 g/mL at 25°C (lit.)
Applications:
- to remove endotoxin from solutions of protein using phase separation technique
- in the extraction and purification of DNA from endotoxin
- in the biphasic extraction of lipoproteins from bacterial cell wall
Additional information
| Size | 500 ml, 1 L |
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