Tween® 20 Purified Proteomic Grade

*Tween 20, also referred as Polyoxyethylenesorbitan monolaurate is a non-ionic detergent, mainly used in membrane research. Membrane proteins are highly sensitive towards perox­ides and carbonyl compounds. While proteins are oxi­dized by peroxides, the Schiff’s base formed during the reaction with carbonyl compounds can influence the proteins function. Also, salts play a role in disturbing the isolation of membrane-bound proteins. Polyoxyethylene type detergents might contain peroxides, carbonyl compounds and salts and peroxide formation is strongly enhanced by light (1, 2). Our purified, proteomic grade 10% Tween 20 has very low levels of unwanted peroxides, carbonyl compounds and salts that reduces the damaging effect of these compounds to the membrane proteins. Extremely low aldehyde & peroxide impurities in the purified detergent enable the highest retention of protein activity. Extremely low conductivity levels in the purified detergent provide low ionic concentrations, which are essential for applications such as electrophoresis.The vials are packed under inert gas, making them extremely stable.

Molecular Formula: C₅₈H₁₁₄O₂₆

Molecular Weight: 1227. 53 g/mol

CAS Number: 9005-64-5

Features:

• Ready-to-use 10% aqueous solution, optimized for isolating proteins and sample preparation
• Reliability – Grants high level of reliability with very low peroxide, carbonyl and salt concentration
  • Typical Analysis: Carbonyl < 200 μM, Peroxide (as H2O2) < 2 ppm, Conductivity <100 μS
• Critical micelle concentration (CMC): 0.06 × 10^-3 M (3)

Applications:

• Solubilizing membrane proteins during extraction of membrane proteins
• Protein detection by UV light absorption, due to its low UV absorbance
• ELISA and immunoblots

*Tween 20 is a registered trademark of Croda International LLC