Description
Antibody Type
Polyclonal Antibody
Uniprot ID
Swiss-Prot: P50552
NCBI Protein: NP_003361.1
Immunogen
Peptide sequence around phosphorylation site of serine 238 (K-V-S(p)-K-Q) derived from Human VASP.
Raised In
Rabbit
Species Reactivity
Human Mouse
Tested Applications
WB IHC Recommended dilution: Predicted MW: 50kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100,
Background / Function
Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation and cell migration. VASP promotes actin filament elongation. It protects the barbed end of growing actin filaments against capping and increases the rate of actin polymerization in the presence of capping protein. VASP stimulates actin filament elongation by promoting the transfer of profilin-bound actin monomers onto the barbed end of growing actin filaments. Plays a role in actin-based mobility of Listeria monocytogenes in host cells. Regulates actin dynamics in platelets and plays an important role in regulating platelet aggregation. Wang HG, et al.
Conjugate
Unconjugated
Storage Buffer
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Modification
Phospho-Ser239
Additional information
| Size | 100?l |
|---|
