The chemical name of X-Gal is X-Gal is 5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside, which is a chromogenic substrate for β-galactosidase and produces an insoluble blue color precipitate by cleaving X-Gal. The insoluble blue color precipitate can easily be detected visually over background. X-Gal is commonly used in molecular biology cloning application to detect the activity of β-galactosidase and in conjunction with IPTG for blue-white bacterial colony screening. It is also used to detect the insertion of foreign DNA into the lacZ region of a plasmid DNA. Insertion of DNA into the lacZ region results in the loss of β-galactosidase activity and the bacteria cells that retain active β-galactosidase will result in characteristic blue colonies. Successful disruption of the lacZα gene disrupts the α-complementation of the β-galactosidase gene and the precipitate does not form, resulting in white colonies.
- X-Gal is used in immune-histochemistry applications.
- It is also the substrate of choice for blue-white selection of recombinant bacterial colonies with the lac+ genotype.
CAS No.: 7240-90-6
Molecular weight: 408.63 g/mol
Molecular Formula: C14H15BrClNO6
Purity: Greater than 99% (HPLC)
Grade: Molecular Biology Grade
Solubility: Clear (2% in DMF)
Biological Activity: Blue color with Lambda, pUC, and M13
Storage/Handling: Store at -20°C. Protected from Light.
PubChem Chemical ID: 619018
InChI Key: OPIFSICVWOWJMJ-AEOCFKNESA-N
Precaution and Disclaimer:
X-Gal is for RESEARCH USE ONLY and harmful if swallowed. Read the product Safety Data Sheet before use in the
research laboratory only.
|Catalog No.||Product Name||Size||Storage Condition|
|10346||X-Gal; 5-bromo-4-chloro-3-indolyl-ß-D-galactopyranoside||100 mg||Desiccated at -20°C|
|10346-1||X-Gal; 5-bromo-4-chloro-3-indolyl-ß-D-galactopyranoside||1 g||Desiccated at -20°C|
100 mg, 1 g